Isolation, identification and molecular characterization of potential probiotic bacterium, Bacillus subtilis PPP 13 from Penaeus monodon
نویسندگان
چکیده
The beneficial effects of probiotics have been attributed to their ability to promote the immunological and nonimmunological defense barrier in the gut, normalization of increased intestinal permeability and altered gut microflora. Twelve different intestinal bacterial colonies were isolated from Black Tiger Shrimp (Penaeus monodon). Among them strain PPP 13 was studied and characterized due to antagonistic properties against three target pathogenic bacterial strains of Vibrio alginolyticus, Vibrio harveyi, Vibrio vulnificus. The strain PPP 13 was identified by morphological, physiological, biochemical characteristics and also by 16S rRNA gene sequence data analysis as Bacillus subtilis. The cultivation time and serum bactericidal activity of nonspecific immunity of Bacillus subtilis PPP 13 were also measured. The antagonistic properties of the experimental strain against target pathogen and the results of serum bactericidal activity of non-specific immunity proved that Bacillus subtilis PPP 13 was a potential probiotic for Penaeus monodon.
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